RNA isolation

Anja Spielvogel a.spielvogel at lb.tu-berlin.de
Wed Aug 25 09:36:54 EST 2004


Hi,

I try to isolate RNA from a filamentous fungi grown on agar plates. When I
grow the fungus on Cellophan it changes growth physiology and expression
pattern. I´d like to avoid growing on Cellophan, but RNA isolation becomes
complicated, because RNA is degraded when I scrape the fungus or try RNA
isolation with whole agar pieces.
Any suggestions?

Thanks in advance

Anja




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