32P will come with a datasheet that tells you the activity and
calibration date and actually will tell you how to do the conversion to
After correcting your counts from the assay date with the calibration
date, you derive the pmol/ml value.
> I am trying to use (32P) orthophosphate to label some E.coli cells, and then
> extract the nucleotides and run them on a thin layer chromatography plates.
> I have questions about how to quantify those radioactive spots? All the
> papers I have seen quantify to the level of pmol/ml, how did they do that?