how to quantify radioactivity?

Austin P. So (Hae Jin) who at what.where
Sat Dec 4 22:37:13 EST 2004

32P will come with a datasheet that tells you the activity and 
calibration date and actually will tell you how to do the conversion to 

After correcting your counts from the assay date with the calibration 
date, you derive the pmol/ml value.


tracy wrote:

> I am trying to use (32P) orthophosphate to label some E.coli cells, and then
> extract the nucleotides and run them on a thin layer chromatography plates.
> I have questions about how to quantify those radioactive spots?  All the
> papers I have seen quantify to the level of pmol/ml, how did they do that?

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