Primer synthesis problems
Duncan Clark
blackhole at abuse.plus.com
Mon Dec 6 05:21:56 EST 2004
Historians believe that in newspost <SOhq6+DCcCtBFAFn at abuse.plus.com> on
Mon, 6 Dec 2004, Duncan Clark <blackhole at abuse.plus.com> penned the
following literary masterpiece:
>Historians believe that in newspost <cor4t6$dp7n$1 at netnews.upenn.edu>
>on Sat, 4 Dec 2004, Paul Shinn <pshinn at force.stwing.upenn.edu> penned
>the following literary masterpiece:
>>Can anyone address these issues? The synthesizer is doing the best it
>>can do. I don't see any problems with it but am I missing something?
>>Could our cloning problems be from bad vector?
>
>Try TA cloning a few PCR products and sequence just the ends.
To be clearer, I mean TA clone before any digest. Raw PCR products.
Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
Duncan Clark
GeneSys Ltd.
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