Primer synthesis problems

Duncan Clark blackhole at abuse.plus.com
Mon Dec 6 05:21:56 EST 2004


Historians believe that in newspost <SOhq6+DCcCtBFAFn at abuse.plus.com> on 
Mon, 6 Dec 2004, Duncan Clark <blackhole at abuse.plus.com> penned the 
following literary masterpiece:
>Historians believe that in newspost <cor4t6$dp7n$1 at netnews.upenn.edu> 
>on Sat, 4 Dec 2004, Paul Shinn <pshinn at force.stwing.upenn.edu> penned 
>the following literary masterpiece:
>>Can anyone address these issues?  The synthesizer is doing the best it
>>can do.  I don't see any problems with it but am I missing something?
>>Could our cloning problems be from bad vector?
>
>Try TA cloning a few PCR products and sequence just the ends.

To be clearer, I mean TA clone before any digest. Raw PCR products.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



More information about the Methods mailing list