Q: multiple bands on Quikchange
blackhole at abuse.plus.com
Wed Dec 8 12:11:48 EST 2004
Historians believe that in newspost
<8b96e0bd.0412080548.753b2a90 at posting.google.com> on Wed, 8 Dec 2004,
Woo Cheol Lee <dreadco at hotmail.com> penned the following literary
>I used PfuTurbo and PfuUltra for quikchange mutagenesis and got
>different results. I have either a small band or a (correct)
>large band with PfuUltra while PfuTurbo yields two bands with
>similar sizes (probably larger one being the correct one?).
>I uploaded the photo of my quikchange results.
>What is the identity of those smaller bands?
Mis-priming by one or more primers?
You appear to have an awful lot of dimers or primers in the reaction
looking at the very small band in the Turbo & Ultra2 photos?
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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