Q: multiple bands on Quikchange

Duncan Clark blackhole at abuse.plus.com
Wed Dec 8 12:11:48 EST 2004


Historians believe that in newspost 
<8b96e0bd.0412080548.753b2a90 at posting.google.com> on Wed, 8 Dec 2004, 
Woo Cheol Lee <dreadco at hotmail.com> penned the following literary 
masterpiece:
>I used PfuTurbo and PfuUltra for quikchange mutagenesis and got
>different results. I have either a small band or a (correct)
>large band with PfuUltra while PfuTurbo yields two bands with
>similar sizes (probably larger one being the correct one?).
>
>I uploaded the photo of my quikchange results.
>
>http://public.fotki.com/dreadco/quikchange/quikchange.html
>
>What is the identity of those smaller bands?

Mis-priming by one or more primers?

You appear to have an awful lot of dimers or primers in the reaction 
looking at the very small band in the Turbo & Ultra2 photos?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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