long range high fidelity PCR
Tim Bullock
tb5v at virginia.edu
Fri Dec 10 09:42:25 EST 2004
Hi,
I'm interested in PCRing out 5-8Kb bands from a BAC to make a targeting
construct for a knockout mouse. Therefore I need a polymerase that is
capable of performing long-range amplifications with very high fidelity.
Yield is less of an issue. Can anyone recommend (from personal experience)
anyone of the myriad of products sold by various companies?
Thanks,
Tim
More information about the Methods
mailing list