long range high fidelity PCR

Tim Bullock tb5v at virginia.edu
Fri Dec 10 09:42:25 EST 2004


Hi,
   I'm interested in PCRing out 5-8Kb bands from a BAC to make a targeting 
construct for a knockout mouse.  Therefore I need a polymerase that is 
capable of performing long-range amplifications with very high fidelity. 
Yield is less of an issue.  Can anyone recommend (from personal experience) 
anyone of the myriad of products sold by various companies?

Thanks,

Tim 





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