long range high fidelity PCR

[Fandango]

"Tim Bullock" <tb5v at virginia.edu> wrote in message
news:cpccjm$e2$1 at murdoch.acc.Virginia.EDU...
> Hi,
>    I'm interested in PCRing out 5-8Kb bands from a BAC to make a targeting
> construct for a knockout mouse.  Therefore I need a polymerase that is
> capable of performing long-range amplifications with very high fidelity.
> Yield is less of an issue.  Can anyone recommend (from personal
experience)
> anyone of the myriad of products sold by various companies?
>
> Thanks,
>
> Tim
>

For this we use Phusion from Finnzymes
http://www.finnzymes.fi/products/pcr/phusion_high_fidelity_dna_polymerase.ht
m

A. Salanti
University of Copenhagen