long range high fidelity PCR
nospam at nospam.com
Tue Dec 14 04:38:17 EST 2004
"Tim Bullock" <tb5v at virginia.edu> wrote in message
news:cpccjm$e2$1 at murdoch.acc.Virginia.EDU...
> I'm interested in PCRing out 5-8Kb bands from a BAC to make a targeting
> construct for a knockout mouse. Therefore I need a polymerase that is
> capable of performing long-range amplifications with very high fidelity.
> Yield is less of an issue. Can anyone recommend (from personal
> anyone of the myriad of products sold by various companies?
For this we use Phusion from Finnzymes
University of Copenhagen
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