dithiothreitol for RT

fabio fabio at fabius.it
Wed Dec 15 16:49:43 EST 2004


I have faund the following mix for RT:
RNA tamplate, random hexameres, --> 70°C x 10'-->ice ,first-strand 
buffer (Invitrogen), dithiothreitol, dNTPs, RNAsin, M-MLV-RT -->37°C x 
1h-->70 °C x 10 ' --> PCR.
The question is: why dithiothreitol ?
Thanks in advance.

Fabio



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