RNA isolation / purity

seg seggewis at gmx.de
Thu Dec 16 09:56:44 EST 2004


Hello!

I´ve a question concerning the purity of isolated RNA:
What about the ratios A260/A280 and A260/A230?

Should they be as high as possible or do values for A260/280 of above 2.1 
and/or values of above 2.4 for A260/A230 indicate contamination, too?

What ranges are the best for each ratio?

What can I do if one value is above a best value?

best regards

Jo 





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