RNA isolation / purity
fabio at fabius.it
Sun Dec 19 16:17:29 EST 2004
seg ha scritto:
> IŽve a question concerning the purity of isolated RNA:
> What about the ratios A260/A280 and A260/A230?
> Should they be as high as possible or do values for A260/280 of above 2.1
> and/or values of above 2.4 for A260/A230 indicate contamination, too?
> What ranges are the best for each ratio?
> best regards
A260/280 ratio display less variability at pH8 than 7, so it might be
useful to evalute nucleic acid molar extintion coefficients at the
Instead to use TE it's better 1-3 mM Na2HPO4 buffer at pH 8,0-8,5
becouse you can treated it with DEPC.
Good ratios are from 1,7-1,8 to 2,2 (I have never seen more than 2,2).
Fabio Di Benedetto
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