DEAE Sepharose "clumping"

Andor J Kiss akiss at life.uiuc.edu
Wed Dec 22 14:32:08 EST 2004


Hello,

    I have a problem with some DEAE Sepharose FF that has clumped.  I've 
tried regenerating it, tried high salt (2M NaCl) tried neutral buffer, 
neutral buffer + high salt.  Nothing seems to work.  Amersham tech support 
is terrible.

Any suggestions?

Andor





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