Copy number of Transgene in Trangenic Mice

Obaid Khan oyk01482 at creighton.edu
Tue Feb 3 17:17:21 EST 2004


I will appreciate if anyone can guide me how to calculate the copy number of
transgenes in mice using southern blot. I have come across some methods on
the net and that are sort of conflicting. I will appreciate if anyone who
has done this will give me some tips and directions on how to spike the
genomic DNA with the transgene and the calculations involved.

Thanks





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