DIG-system/CSPD/high background after stripping of probe

Sandro Conrad sandro.conrad at freenet.de
Thu Feb 5 04:30:54 EST 2004


sorry, no idea.
maybe another here.

Sibylle schrieb:
> Hi!
> 
> It´s some weeks now that our DIG-detection system (Boeringer/Mannheim)
> doesn´t work anymore. Our problem is the stripping of a probe before
> re-hybridization it with another (stripping is absolutely essential
> for our work and worked properly in the last 5 years). Although Ab-
> and Substrate-Solution are always rel. new, the background is to dark
> now.
> The membrane which we use is HybondN (Amersham).
> We checked the membrane (whose composition, besides, was changed
> within the last 5 years), all solutions for hybridization were made
> new, blocking-buffer was checked, time of stripping was increased.
> 
> Did "Tropix" change the composition of CSPD?
> 
> If anybody knows anything, please let us know!
> 
> Thanks in advance,
> Sibylle




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