PCR buffer with Tris-HCl and Ammonium sulfate (4:1)-does it exist?

Elisabet W elisabet.witso at chello.no
Thu Feb 5 08:04:08 EST 2004


Why is it Ammonium sulfate in my nested PCR mix?

Can you by a PCR buffer which contains Ammonium sulfate together with
Tris-HCL (pH 8.5), when the consentration is 4:1?

Or do I have to make these reagents by myself? 

I use primers that are degenerated and contains deoxyionisine, if that
can help.

Elisabet W



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