HUH7 cells maintainance PROBLEM

redeamer alion85 at
Fri Feb 6 07:33:24 EST 2004

Hi guys!

i have a very big problem with maintaining Huh7 human hepatocarcinoma
cell line, i use IMDM as medium with 1XPen-Str and 10% Foetal Calf
Serum... I trypsinize them and afterwards they are not really happy:((
As for their morphology: interesting cells almost transparent....
something is wrong:(

Can you share some really good protocol for maintaining Huh7 and their
electroporation conditions (both with DNA and RNA), as well as their
splitting techniques... i feel that trypsinization must becrucially
transformed or replaced with some other method.... may be its better
for them to grow on a plain flask?

Any suggestions are highly appreciated,

thank you

---------------> Drew

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