HUH7 cells maintainance PROBLEM

EK nobody at
Fri Feb 6 16:48:10 EST 2004

Check Invitrogen website:


"redeamer" <alion85 at> wrote in message
news:a86fd891.0402060433.3b3937a4 at
> Hi guys!
> i have a very big problem with maintaining Huh7 human hepatocarcinoma
> cell line, i use IMDM as medium with 1XPen-Str and 10% Foetal Calf
> Serum... I trypsinize them and afterwards they are not really happy:((
> As for their morphology: interesting cells almost transparent....
> something is wrong:(
> Can you share some really good protocol for maintaining Huh7 and their
> electroporation conditions (both with DNA and RNA), as well as their
> splitting techniques... i feel that trypsinization must becrucially
> transformed or replaced with some other method.... may be its better
> for them to grow on a plain flask?
> Any suggestions are highly appreciated,
> thank you
> ---------------> Drew

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