Huh7 and acetone;)

P.C. PC at no.email.sorry
Sun Feb 8 00:13:09 EST 2004


acetone is a very harsh precipitant. I would expect almost any protein 
to lose activity. It might work for very soluble, small proteins, may be...

are you sure this is the best way?


Peter

EK wrote:
> "redeamer" <alion85 at hotmail.com> wrote in message
> news:a86fd891.0402070046.6cd7b9f4 at posting.google.com...
> 
>>Thanks a lot, Emir!
>>
>>very useful information on Invitrogen site...
> 
> 
> Glad you found it useful :-
> 
>>however, acetone has a protein denaturing property...
>>
> 
> That means it would not hurt to have a bit of cofactor metal dissolved in
> the reconstitution buffer. I am OK with that.
> -
> Emir
> 
> 




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