Huh7 and acetone;)

[redeamer]

Unfortunately, the simpliest and easiest does not work for my
proteins:) they are all membrane-bound, purifying them with Ni-NTA, S-
and Q-sepharose :))

you are lucky, Emir!:)

However, wasn't Alkaline Phosphatase a peripheral protein, membrane
bound?

by the way, do not forget:

------> science should be as simple as possible, but not simpler
------> |||||||||||||||||||Albert Einstein||||||||||||||||||||||

--->Cheers,
         Drew