``||´´_Linear_``||´´_Conformational_``||´´_Epitope_``||´´_Retrieval

redeamer alion85 at hotmail.com
Sun Feb 8 16:15:57 EST 2004


Hi guys!

i have one, may be stupid, question: 

If an antibody recognizes linear epitope, is there any sense to check
this antibody on native epitope target? I mean if i do an epitope
mapping and have a signal on my western blot (that i did from SDS
PAG), should i do immunoprecipitation and then western to retrieve a
possible conformational epitope for the same protein?

I think that the answer is yes, but this is somekind of intrinsic
feeling, that i cannot evolve now:(

so i need YOUR help :)

THANK YOU in advance,


--------> Drew



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