Synthesize your own oligo dT?

Paul Shinn pshinn at force.stwing.upenn.edu
Thu Feb 12 14:15:59 EST 2004


   Is there anything special about the oligo dT supplied in RT-PCR
kits for first strand DNA synthesis?  We have our own oligo synth
machine we use for PCR and sequencing primers.  Is there any reason
why I couldn't make a 20mer of dT-CE phosphoramidite and use it
for the first strand reaction?

				Thanks, Paul




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