Synthesize your own oligo dT?

Nick Theodorakis nick_theodorakis at
Thu Feb 12 22:43:56 EST 2004

On Thu, 12 Feb 2004 19:15:59 +0000 (UTC), Paul Shinn
<pshinn at> wrote:

>   Is there anything special about the oligo dT supplied in RT-PCR
>kits for first strand DNA synthesis?  We have our own oligo synth
>machine we use for PCR and sequencing primers.  Is there any reason
>why I couldn't make a 20mer of dT-CE phosphoramidite and use it
>for the first strand reaction?

I get my oligo dT for RT synthesized as an oligo -- it's a lot cheaper
than buying "oligo-dT" for some reason. 


Nick Theodorakis
nick_theodorakis at
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu 

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