Alternatives to Laemli?

MICHAEL L SULLIVAN mlsulliv at facstaff.wisc.edu
Mon Feb 16 17:17:28 EST 2004


                                                     
>                                                               lab 
> gels.  Since there is
> > no stack with this one, sample volumes need to be small to get 
> tight bands.
> 
> There is no reason not to include the stacker. 
> 

Is there a point to including a stacking gel here, since the pH difference wouldn't be that great between the stacking/resolving gels?  I thought that was the point of a stacking gel-- having the pH difference at the interface of the resolving/stacking gel.  Of course, I've been puzzled how one can have precast gels, since presumably the pH difference couldn't be maintained upon storage, so maybe I'm wrong on this.

Mike

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