Insoluble GST fusion protein help

Michael O'Hare mohar052removethis at
Tue Feb 17 11:13:02 EST 2004

Hi.  I need help purifying a highly insoluble GST-fusion protein.  I 
don't neen lots of it, just enough to serve as substrate in a kinase 
assay.  When I test my extracts from IPTG-induced vs non-induced I don't 
see any differences in the banding pattern.   Any advice would be 

Even better, if anyone has any purified E2F1, E2F2, and E2F3 protein I 
would be very grateful if you could give me a small aliquot.


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