Alternatives to Laemli

ahernan at cica.es ahernan at cica.es
Wed Feb 18 03:25:53 EST 2004


This message seems to have been lost in e-space. I repost it.


Thanks to all,

I think I'll try Mike Sullivan's suggestion but with a stacking on 
top (maybe 3% ).
As for the presence of SDS, the protein and the RNA are (hopefully!) 
crosslinked, so, if there are no problems with size, the complex 
should enter the gel and stay put.
Anyone heard about a Tris-Acetate system, anyway?
Cheers

Agustin
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