Removal of DNA after isolation of Total nucleic acid?
Dr. Hiranya S. Roychowdhury
hroychow at nmsu.edu
Fri Feb 20 12:11:20 EST 2004
You can buy RNAse-free DNase from a good supplier.
It is also better if you used low-pH phenol extraction which removes most of
the DNA from the prep. Thre are also kits available from various suppliers that
will leave you only with RNA from a total NA prep.
Quoting Elisabet W <elisabet.witso at chello.no>:
> I need to remove the DNA after isolation of Total nucleic acid from
> stool after isolation on MagnaPure LC, Roche. How can I do that easy
> and safe without destruction of any RNA in the sample?
> Elisabet W
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
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