band separation prob. on agarose gels

Ferdinando Pucci brotizolam at
Sun Feb 22 08:51:42 EST 2004

On Mon, 16 Feb 2004 18:14:31 GMT
"Kyle Legate" <legatek at> wrote:

> I'm trying to do a simple sticky-sticky cloning step using EcoRI and
> NotI. I cut 5 microg. of each plasmid. One plasmid yields 4.3kb and
> 2.5kb bands, and the other plasmid yields 3.5kb and 1.6 kb bands. I
> digest both plasmids and separate the bands on a 1% agarose gel in TAE
> at 5V/cm. I

Did you inactivate the enzymes? How many wells did you used?

The propriety of some persons seems to consist in having improper
thoughts about their neighbours.
		-- F.H. Bradley

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