SUMO interaction in a Yeast Two Hybrid Assay
ucgatan at socrates-a.ucl.ac.uk
Mon Feb 23 06:33:57 EST 2004
On 20 Feb 2004, Andrew wrote:
> In my reading I've come across many examples of people utilizing the
> Y2H assay to demonstrate interaction between their protein and SUMO.
> Some investigators conclude based on their interaction data that this
> is SUMO conjugation;
You mean they use SUMO as bait and their protein as prey, and if they get
a positive result, they conclude that their protein is SUMOylated? Unless
i'm missing something, that's pure madness.
Could you give a couple of examples of such papers? Maybe i've
misunderstood what they're doing.
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL
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