stable expression in 293

Han noone at nospam.invalid
Tue Feb 24 20:39:42 EST 2004


hubahopp at gmx.de (Wolfgang Schechinger) wrote in
news:3.0.6.32.20040224235031.00c67d68 at pop.gmx.net: 

> If your insert and the construct are ok, maybe the cells don't like
> your protein. You could try other cells, even 3T3 might express your
> CMV driven plasmid. 
> 
> You might check a recent paper by Steve Roffler on a expressionmethod
> that works like a charm:
> http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed
> &list _uids=14725509&dopt=Abstract
> 
> Wo
<snip>

I emailed Dr Roffler for a copy of the paper.  Sounds very interesting.

-- 
Best regards
Han 
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