K+ SDS precipitation

EK nobody at elnino.com
Tue Jan 6 18:32:13 EST 2004


> > Don't wash with acetone then and just resuspend in say 2x SDS loading
> > buffer.
> >
> If you don't wash with organnis solvent, you will have to supplement your
> SDS-PAGE buffer with Tris base to counter the acidity. Easy to do if you
> remember that blue=good and yellow=bad.

Usually, you don't have to worry about adjusting pH, because protein pellets
are small and most of TCA can be efficiently sucked out. The volume of the
pellet will be too small to cause pH change in the SDS loading buffer,
especialy if you use 2x concentrated one.






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