K+ SDS precipitation

Schwabe, TME tatjana.schwabe at ruhr-uni-bochum.de
Thu Jan 8 11:49:48 EST 2004


I don't know whether this is an option for you, but we titrate our HEPES 
with NaOH. Would this be possible in your extraction method?


Good Luck,
Tatjana

redeamer wrote:


> I ve prepared eukaryotic cell extract using a buffer, containing
> potassium (Hepes-KOH pH 7.9). I need this extract for western blots.
> As expected
> when i add protein loading buffer (SDS inside) all the stuff
> precipitates
> on entering the well of polyacrylamide gel :(.... I run the gel.. and
> had
> only part of desired signals on western (maybe because of material
> insufficiency)....
> Drew




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