FW: migration of DNA compared to RNA in TBE-Urea polyacrylamide
morten at binf.ku.dk
Tue Jan 13 05:01:28 EST 2004
Deanne Bell wrote:
> -----Original Message-----
> From: Morten Lindow [mailto:morten at binf.ku.dk]
> Sent: Monday, January 12, 2004 5:27 AM
> To: methods at hgmp.mrc.ac.uk
> Subject: migration of DNA compared to RNA in TBE-Urea polyacrylamide
> Any experienced biochemist out there who can help answer this?
> In a denaturing TBE-Urea polyacrylamide gel, does DNA migrate at the
> same speed as RNA?
> In other words: Can I use a DNA size marker to get an idea about the
> size of RNA samples? Will the urea denature the DNA-helix and make it
> single stranded?
> My RNA is supposed to be only around 20 nt, so cannot use a RNA ladder
> unless I do radiolabelling.
> Just an idea - can you use a sequencing reaction? That is what I run
> with my microsatellites on a urea PAGE
That's not a bad idea. But I wonder if the mini-gel will resolve 1 base
differences. I'll try.
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