FW: migration of DNA compared to RNA in TBE-Urea polyacrylamide gels

Morten Lindow morten at binf.ku.dk
Tue Jan 13 05:01:28 EST 2004


Deanne Bell wrote:
> -----Original Message-----
> From: Morten Lindow [mailto:morten at binf.ku.dk] 
> Sent: Monday, January 12, 2004 5:27 AM
> To: methods at hgmp.mrc.ac.uk
> Subject: migration of DNA compared to RNA in TBE-Urea polyacrylamide
> gels
> 
> Any experienced biochemist out there who can help answer this?
> 
> In a denaturing TBE-Urea polyacrylamide gel, does DNA migrate at the 
> same speed as RNA?
> 
> In other words: Can I use a DNA size marker to get an idea about the 
> size of RNA samples? Will the urea denature the DNA-helix and make it 
> single stranded?
> 
> My RNA is supposed to be only around 20 nt, so cannot use a RNA ladder 
> unless I do radiolabelling.
> 
> cheers,
> 
> Morten
> Copenhagen
> 
> 
> Hi 
> Just an idea - can you use a sequencing reaction?  That is what I run
> with my microsatellites on a urea PAGE
> Dee
That's not a bad idea. But I wonder if the mini-gel will resolve 1 base 
differences. I'll try.

Morten


> ---




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