Does linearisation of DNA help stable trasfection

Morten Lindow morten at binf.ku.dk
Tue Jan 13 06:41:30 EST 2004


sriadi at hotmail.com wrote:
> Does some one know if linearization of Plasmid DNA will increase the chances of getting the stable clones. I am using exgen500 from fermentas as the vector which is a linear Polyethylenimine. 
> Thank you very much in advance.
> 
> 
> 
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0
I've seen several protocols for generation of stable cell lines that 
includes linearization. So I guess some people thinks it helps. Not sure 
why though - perhaps because you get to choose where the vector is cut, 
instead of a more random cut that might break your ORF?

Morten




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