Does linearisation of DNA help stable trasfection
morten at binf.ku.dk
Tue Jan 13 06:41:30 EST 2004
sriadi at hotmail.com wrote:
> Does some one know if linearization of Plasmid DNA will increase the chances of getting the stable clones. I am using exgen500 from fermentas as the vector which is a linear Polyethylenimine.
> Thank you very much in advance.
I've seen several protocols for generation of stable cell lines that
includes linearization. So I guess some people thinks it helps. Not sure
why though - perhaps because you get to choose where the vector is cut,
instead of a more random cut that might break your ORF?
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