SAGE and gel purification of ditags

Wolfgang Schechinger hubahopp at
Wed Jan 14 19:18:36 EST 2004


what do you think that heating will improve - killing NlaIII? 

Just curious,


At 12:10 14.01.2004 -0800, Carl wrote:
>I'm doing SAGE and it seems like many of my ditags are jamed in the
>well when I purify them after the NlaIII digestion.
>I would like to know if someone ever tried to heat the sample just
>before putting it on the acrylamide gel after the NlaIII digestion and
>if it improves the yield in purified ditags.
Wolfgang Schechinger


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