Rapid DNA extraction methods

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Sat Jan 17 12:32:48 EST 2004

A rather toxic method is the benzylchloride extration that can be applied to 
small sample masses and can be done on a number of samples at a time.  It does 
damage DNA somewhat (strand breaks), but is generally suitable for PCR 
(analytical).    Other than this, I would think that a simple extraction in 
Tris buffer or PBS using a little SDS (0.1% or less) should yield some DNA for 
diagnostics from filter paper or cotton swabs.  

Quoting Drew <andy.hopwood1 at ntlworld.com>:

> I'm looking for a rapid yet robust DNA extraction method for
> application to small samples (<1 ul blood collected on paper or
> cotton) It needs to be 30 min or less and ideally give me good quality
> PCR-friendly DNA.  A lot to ask I know. Anybody out there up for the
> challenge?
> Drew

Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003

More information about the Methods mailing list