anti-ubuquitin recommendation wanted

Henk Veldman ln.uza.oruen at 2-namdleV.H
Fri Jul 2 03:01:28 EST 2004


Man-Ho Chu schreef:
> 
> Hi all,
> Can anyone give me recommendation of a good ubiquitin antibody?  (Low
> background, high specificity, and suitable for WB and IP.)
> Thanks in advance.
> 
> Man-Ho Chu

Beware:
Anti-ubiquitin antibodies often are specific for either conjugated or
unconjugeted ubiquitin! I expect you might prefer different
specificities for immunohistochemistry and Western.

We used a polyclonal (to conjugated ubiquitin) from Dako that worked
fine in immunohistochemisty.

Henk



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