Old expression vectors

Nick Theodorakis nick_theodorakis at hotmail.com
Wed Jul 7 22:25:15 EST 2004


On Wed, 7 Jul 2004 12:19:38 +0000 (UTC), lhom at OCF.Berkeley.EDU (Louis
Hom) wrote:

>
>I was thinking about pUC or Bluescript, but I was hoping to avoid the 
>problem of the MCS being inside the lacZ ORF -- I'd like to have it start 
>at my ATG and not have the extra start site or N-terminal residues.
>-- 

What if you gave your ORF a good strong S-D sequence by its own ATG?

Nick


-- 
Nick Theodorakis
nick_theodorakis at hotmail.com
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu 



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