Red/ET recombination

Tam tomatik_t at mail.ru
Mon Jul 12 07:44:11 EST 2004


Have anyone tried Gene Bridges Red/ET recombination kit?

I got some questions:

1. If I can't shake my bacteria at 1100 rpm (don't have such a
powerful shaker), can I grow them longer until desired OD 0.2?  What
could you recomend? May be aerate them more by growing in a larger
volume?
2. How many times you add dH2O, when you make electrocompetent cells?
3. Have you tried counter-selection kit? Does it work?

Thank you in advance,

Tam



More information about the Methods mailing list