CYP2D6*4 allele and assy by demand kit from Applied biosystems

Brian O'Mahony bomahony at imim.es
Wed Jul 14 04:27:31 EST 2004


bomahony at imim.es (Brian O'Mahony) wrote in message news:<fdbf07.0407060249.4e481bc9 at posting.google.com>...
> Hi,
> 
> I have been doing CYP2D6 genotyping for alleles *6, *3 and *4. For 3
> and 6 its works but for 4 no. I use 5ul rxn volume and my system is
> the ABI7500 sequence detection. Even the control DNA doesn't work. I
> have it at concentrations of 50ng and 100ng/rxn. Experimental DNA
> ranges from 20 ng-800ng/rxn. Even at the higher end of the range the
> rxn doesn't work. I tried changing the anneling temperature from 60ºC
> (standard) to 58ºC. Can anybody help me? Or is anyone having problems
> with this paricular product. They sent me new reagents but it still
> doesn't work.
> 
> Thanks
> 
> Brian

Once again can nybody help me. I would really like to start on this!!

Thanks



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