Cell disruption without sonication

voyager ckamei at terra.com.br
Sun Jul 18 08:35:37 EST 2004


Help !!!!

I am expressing my protein in E. coli BL21, and I am looking for
another way to disrupt the cells without sonication
 Does anyone have
a protocol ?!?!?!  I tried lyzosyme followed by frezze-defrezze
methodology, but the separation of pellet, supernatant and debrit was
terrible
 I needed to centrifugate several times, so I am not sure
about the integrity of my protein
 I am growing a culture of 50 mL.

Please, send me some advice !!! Thanks everybody,

Claire



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