Cell disruption without sonication

Louis Hom lhom at OCF.Berkeley.EDU
Sun Jul 18 20:01:18 EST 2004


I like to resuspend the cells in Tris + protease inhibitor (PMSF) and 
treat with lysozyme for about 20 minutes.  Then add Triton X-100 to 0.5% 
and MgCl2 to 5 mM.  The Triton gives nice lysis, while the MgCl2 helps tie 
up (or aid in the digestion of) the DNA, providing a nice solid pellet 
(say, 20-30 minutes at 3000xg).
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    



More information about the Methods mailing list