digestion of PCR reaction
donot at nospam.com
Wed Jul 21 08:04:05 EST 2004
I have a basic question.
Is it possible to restriction digest a PCR product directly from the
reaction mix containing a proofreading enzyme. I am afraid that the enzyme
(called phusion) during the digest will remove the protruding ends so I
can't clone it directly into a cut vector?
Usually I PCR purify it on a column, I just wondered whether this step was
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