digestion of PCR reaction

Fam donot at nospam.com
Wed Jul 21 08:04:05 EST 2004


Dear NG
I have a basic question.
Is it possible to restriction digest a PCR product directly from the
reaction mix containing a proofreading enzyme. I am afraid that the enzyme
(called phusion) during the digest will remove the protruding ends so I
can't clone it directly into a cut vector?
Usually I PCR purify it on a column, I just wondered whether this step was
necessary. Thanks





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