XbaI diestion of PCR products

Raymond Dalgleish ray at le.ac.uk
Thu Jul 22 07:00:32 EST 2004


We are having problems digesting PCR-amplified DNA with XbaI. It's for RFLP
testing and the site is well within the amplified fragment, not at the end.
The digests are always partial, and cleaning the DNA on Qiagen only seems to
give a marginal improvement. The fragment digests correctly with PvuII
without any clean up.

Information on the Pall website (http://www.pall.com/924_25460.asp - see end
of article) alludes to the issue.

Does anybody else have this problem with XbaI, and how do you work around
it?

Thanks,

Raymond





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