XbaI diestion of PCR products
Dr. Hiranya S. Roychowdhury
hroychow at nmsu.edu
Thu Jul 22 10:10:06 EST 2004
XbaI, in my experience, is not one of the finnicky enzymes. It is possible
that you have been just unfortunate enough to have a misincorporation at the
presumptive XbaI site in the amplicon; isn't it?
Quoting Raymond Dalgleish <ray at le.ac.uk>:
> We are having problems digesting PCR-amplified DNA with XbaI. It's for RFLP
> testing and the site is well within the amplified fragment, not at the end.
> The digests are always partial, and cleaning the DNA on Qiagen only seems
> give a marginal improvement. The fragment digests correctly with PvuII
> without any clean up.
> Information on the Pall website (http://www.pall.com/924_25460.asp - see
> of article) alludes to the issue.
> Does anybody else have this problem with XbaI, and how do you work around
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
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