Mysteriously Disappearing DNA

Shelley.Sandiford at lrcc.on.ca Shelley.Sandiford at lrcc.on.ca
Wed Jul 28 17:23:52 EST 2004



Just a quick question as I am new to cloning.  I have a sample of bacteria that
were successfully transformed with my vector/gene of interest.  The problem that
I am
having is that when I attempt to grow a new sample from my positive clone, the
vector (along with the gene) appears to be diluted out (i.e. something
ampicillin
resistant is growing, but no longer appears to contain the original vector).

Has anyone ever experienced this kind of "dilution" and/or have any suggestions
as to what might be happening (contamination, etc)?  I am open to any ideas.

Shelley Sandiford



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