Why DTT in RT-PCR?

Fandango nospam at nospam.com
Wed Jun 2 03:59:23 EST 2004


I once saw an article (in Biotechniques, I think) that when doing real time
RT-PCR you got the highest CT values if you omitted DTT! So it might not be
necessary to use it.

"Philipp" <philipp.bassler at gmx.de> wrote in message
news:6b2d9083.0405300849.5a65e145 at posting.google.com...
> Hi!
>
> I have a short question: Why is ist necessaire to use DTT-Solution for
> a RT-PCR? What does it result in?
> Thank you very much for your answer!
>
> Philipp





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