Why DTT in RT-PCR?

Fandango nospam at nospam.com
Thu Jun 3 02:21:45 EST 2004

Sorry, I mean the best CT value, which is of cause the lowest value!
"Fandango" <nospam at nospam.com> wrote in message
news:c9k4ci$hka$1 at news.net.uni-c.dk...
> I once saw an article (in Biotechniques, I think) that when doing real
> RT-PCR you got the highest CT values if you omitted DTT! So it might not
> necessary to use it.
> "Philipp" <philipp.bassler at gmx.de> wrote in message
> news:6b2d9083.0405300849.5a65e145 at posting.google.com...
> > Hi!
> >
> > I have a short question: Why is ist necessaire to use DTT-Solution for
> > a RT-PCR? What does it result in?
> > Thank you very much for your answer!
> >
> > Philipp

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