band-shift

jens appel jappel at bot.uni-kiel.de
Thu Jun 3 05:57:38 EST 2004


We used dig labelled oligonucleotides in a band-shift assay. Our problem
is, that the oligonucleotides seem to get destroyed when adding our
protein crude extract ( the signal strength of the probe without protein
is much stronger than the signal from probes with added proteins). Does
anyboby have an idea how to avoid this proplem?
Kirstin

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