Removal of antibodies in Co-IP
Simon T. Buttgereit
tbutt at web.de
Fri Jun 4 03:20:34 EST 2004
Dr Engelbert Buxbaum wrote:
> Either crosslink the antibody to a carrier (e.g. agarose beads) or use
> denaturing isoelectric focussing instead of SDS-PAGE for separation.
Have tried ist, but activity of antibody went down dramatically.
> What kind of detection are you using?
I just want to cut out differential protein bands between two induction
timepoints for MALDITOF. 2D-PAAG should be performed later.
With metabolically labelled 35S or
> with 125I labelled proteins the presence of the non-radioactive Ig chain
> would not interfere with detection.
thats right, but they MALDI-Guys don´t like radioactivity...
I have found some kits for antibody removal. Have an idea how they work?
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