Inositol Triphosphate (IP3) measurement in cells

[redeamer]

seedelm at yahoo.com (S.E.) wrote in message news:<6b3dbce8.0406011133.1dbaaf30 at posting.google.com>...
> We were using PerkinElmer's Inositol 1,4,5 Triphosphate RIA Kit (Cat #
> NEK-064) to measure IP3 extracted from tissues.  We now want to
> perform the same experiment on cells.
> 
> The problem is that PerkinElmer's kit is backordered indefinitely. 
> Now what?  Does anybody have a protocol that describes how to do this
> the old-fashioned way?  Is there another manufacturer who has a
> similar kit?
> 
> Any help, advice, input etc.  is very much appreciated.
> 
> Thanks, 
> Stephanie


Hi Spephanie!

Wasnt that IP3 concentration was correlated with Ca2+ released from ER
or SER inside the cell??? Ca2+ are readily detected, no prob with
that... If you use somekind of stimulation than you can include just
additional control into your experiment set.... Hope this helps


--> Drew