Inositol Triphosphate (IP3) measurement in cells
alion85 at hotmail.com
Sat Jun 5 18:52:38 EST 2004
seedelm at yahoo.com (S.E.) wrote in message news:<6b3dbce8.0406011133.1dbaaf30 at posting.google.com>...
> We were using PerkinElmer's Inositol 1,4,5 Triphosphate RIA Kit (Cat #
> NEK-064) to measure IP3 extracted from tissues. We now want to
> perform the same experiment on cells.
> The problem is that PerkinElmer's kit is backordered indefinitely.
> Now what? Does anybody have a protocol that describes how to do this
> the old-fashioned way? Is there another manufacturer who has a
> similar kit?
> Any help, advice, input etc. is very much appreciated.
Wasnt that IP3 concentration was correlated with Ca2+ released from ER
or SER inside the cell??? Ca2+ are readily detected, no prob with
that... If you use somekind of stimulation than you can include just
additional control into your experiment set.... Hope this helps
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