Ideas for presentation?

Deanne Bell dbell at fresno.ars.usda.gov
Mon Jun 14 12:19:37 EST 2004


Hey Trond
Type "dna extraction from onion" into google and there are several
protocols to choose from.

TTFN
Dee Bell


-----Original Message-----
From: owner-methods at hgmp.mrc.ac.uk [mailto:owner-methods at hgmp.mrc.ac.uk]
On Behalf Of Trond Erik Vee Aune
Sent: Monday, June 14, 2004 1:53 AM
To: methods at hgmp.mrc.ac.uk
Subject: Re: Ideas for presentation?

Thank you all,

Does any of you have protocol for DNA extraction from onion? And why
onion?

Trond Erik

DavidMicklem wrote:

> In article <cabk3a$vmg$1 at orkan.itea.ntnu.no>, Trond Erik Vee Aune
> <trondaun at biotech.REMOVETHISBEFOREREPLYING.ntnu.no> wrote:
> 
> 
>>Hi,
>>
>>I'm going to participate at a "Science Day" in the city where I live, 
>>and have been asked to make an interactive presentation for children 
>>from 10 to 19 where the topic is gene technology/microbiology. I can 
>>borrow some equipment from the lab where I work (general molecular 
>>biology lab). Do any of you have any ideas what I could do? Ideally it

>>should involve the children and trigger their curiosity while being
fun 
>>at the same time.
>>
>>Trond Erik
> 
> 
> Could they make a rather quick and dirty DNA prep from eg onions?
Spool
> out the DNA from ethanol (hmm, expensive in Norway, and maybe not
> allowed for children??). Get to take the DNA home with them....
> 
> Some kind of colour-changing thing using eg lacZ??
> 
> Showing cell under microscope - their own cheek cells?? 
> 
> GFP (RFP, CFP, YFP) is always fun in gene tech - maybe you could have
> multi-coloured bacterial preps? You can excite it using BLUE light, so
> no need for UV. (e.g. a blue LED works OK, or a DarkReader, with
orange
> filter).
> 
> Um, er, you don't have a GFP mouse running around anywhere do you :-)
> 
> David

-- 
Trond Erik Vee Aune
Department of Biotechnology
Norwegian University of Science and Technology
Email: trondaun at REMOVETHISBEFOREREPLYING!!biotech.ntnu.no
http://www.biotech.ntnu.no/molgen

- Must be sad being a dyslectic, agnostic insomniac, lying
   awake during the night, wondering if there really is a dog

---



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