slot-blot

Marius Banholzer marius.banholzer at uniklinik-freiburg.de
Wed Jun 16 07:38:41 EST 2004


I´m doing DNA-slot-blots. The DNA is denatured by cooking with NaOH for 
3 minutes at 95 degrees. For blotting a Nylon-membrane is used. The 
problem is, that the probes are not sucked through the membrane. Even 
the washing buffer won´t go through.
The vacuum is ok. Please help!

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